吉林建筑大学学报

针对供水系统耐氯性细菌(Chlorine-resistant bacteria, CRB)带来的水质安全问题，以北方地区某城市供水管网中筛选的耐氯性菌株为研究对象，研究了耐氯性细菌菌株的生化特性、氯消毒和紫外线消毒方式下耐氯性细菌菌群的灭活效果及抗生素抗性。结果显示：1年内经3次加氯筛选得到24株CRB,分为芽孢杆菌、单胞菌和大肠杆菌3类。使用3 mg·L^-1,6 mg·L^-1浓度氯消毒20 min时，3类CRB的灭活效率分别为2.3 lg, 2.4 lg, 2.6 lg和2.6 lg, 2.8 lg, 2.9 lg;当紫外线消毒浓度为80 mJ·cm^-2时，CRB的灭活效率分别为0.96 lg, 1.04 lg, 1.12 lg。分离的CRB对青霉素和四环素产生抗性，而对阿奇霉素的抗性较低。北方城市供水系统中CRB对紫外线消毒和氯消毒产生一定的抗性，需要探索多种消毒措施协同控制城市供水系统中微生物，确保城市管网水质的安全。

In order to view of the water quality problems caused by chlorine-resistant bacteria(CRB) in the water supply system, the biochemical characteristics of chlorine-resistant strains, the inactivation efficiency of chlorine resistant bacteria under chlorine resistant disinfection and ultraviolet disinfection and antibiotic resistance were studied by taking the chlorine resistant strains isolated and screened in the water supply pipe network system of a city in northern China as the research object.The results showed that a total of 24 chlorine tolerant strains were screened within one year, which were divided into three categories: Bacillus, Monomonas and Escherichia coli, and strong resistance to chlorine and ultraviolet disinfection was produced.When they were inactivated for 20 min, the inactivation of Cl₂ disinfection with the concentration of 3 mg ·L^-1 on CRB were 2.3 lg, 2.4 lg and 2.6 lg, respectively.Under the same inactivated time with Cl₂ disinfection with the concentration of 6 mg ·L^-1,the inactivation rate of three kind of CRB reached to 2.6 lg, 2.8 lg and 2.9 lg, respectively.In addition, when the dose of ultraviolet disinfection was 80 mJ ·cm^-2,the inactivation efficiency of three kinds CRB had been reached to 0.96 lg, 1.04 lg and 1.12 lg, respectively.The resistance to penicillin and tetracycline of the CRB were lower than the resistance to rifampicin.All these results had been showed that CRB in northern urban water supply systems had certain resistance to ultraviolet light and chlorine disinfection, and the antibiotic resistance genes were not completely treated.Meantime, it was necessary to explore a variety of disinfection measures to control microorganisms in urban water supply systems to ensure the safety of drinking water and provide technical support.